KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Viperin (MaP.VIP) | Wang et al., 2007 | N/A |
| GRP94 | Enzo Life Sciences | Cat#ADI-SPA-851; RRID: AB_10615790 |
| GAPDH | Proteintech | Cat#60004–1-Ig; RRID: AB_2107436 |
| Phospho-4E-BP1 (Ser65) | Cell Signaling Technology | Cat#9451; RRID: AB_330947 |
| 4E-BP1 | Cell Signaling Technology | Cat#9644; RRID: AB_2097841 |
| Phospho-S6 Ribosomal Protein (Ser235/236) | Cell Signaling Technology | Cat#4857; RRID: AB_2181035 |
| S6 Ribosomal Protein (S6) | Cell Signaling Technology | Cat#2217; RRID: AB_331355 |
| eIF2α | Cell Signaling Technology | Cat#5324; RRID: AB_10692650 |
| phospho-eIF2α (Ser51) | Cell Signaling Technology | Cat#3597; RRID: AB_390740 |
| phospho-p38 (Thr180/Tyr182) | Proteintech | Cat#9211; RRID: AB_331641 |
| p38 | Proteintech | Cat#9212; RRID: AB_330713 |
| phospho-JNK (Thr183/Tyr185) | Proteintech | Cat#4668; RRID: AB_823588 |
| Flavivirus envelope protein | Millipore | Cat#MAB10216; RRID: AB_827205 |
| GCN2 | Cell Signaling Technology | Cat#3302; RRID: AB_2277617 |
| phospho-GCN2(T899) | Abcam | Cat#ab75836; RRID: AB_1310260 |
| PERK | Cell Signaling Technology | Cat#3192; RRID: AB_2095847 |
| HRI | Proteintech | Cat#20499–1-AP; RRID: AB_10697665 |
| PKR | Cell Signaling Technology | Cat#3072; RRID: AB_2277600 |
| ZAK | Bethyl Laboratories | Cat#A301–993A; RRID: AB_1576612 |
| goat anti-mouse IgG, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A-11017; RRID: AB_2534084 |
| goat anti-mouse IgG, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#A-21236; RRID: AB_2535805 |
| goat anti-rat IgG, Alexa Fluor 546 | Thermo Fisher Scientific | Cat#A-11081; RRID: AB_2534125 |
| goat anti-rat IgG, Alexa Fluor 594 | Thermo Fisher Scientific | Cat#A-11007; RRID: AB_10561522 |
| goat anti-rabbit IgG, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#A-21244; RRID: AB_2535812 |
| goat anti-mouse IgG-HRP | Jackson ImmunoResearch Laboratories | Cat#115–035-146; RRID: AB_2307392 |
| goat anti-rabbit-HRP | Jackson ImmunoResearch Laboratories | Cat#111–005-144; RRID: AB_2337919 |
| goat anti-rat IgG-HRP | Jackson ImmunoResearch Laboratories | Cat#112–005-167; RRID: AB_2338101 |
| Bacterial and virus strains | ||
| NEB 5-α Competent E. coli (High Efficiency) | New England Biolabs | Cat#C2987 |
| Zika virus Cambodia/FSS13025 strain | World Reference Center for Emerging Viruses and Arboviruses at University of Texas Medical Branch at Galveston | NCBI:txid2316109 |
| NanoLuc reporter ZIKV | Baker et al., 2020 | N/A |
| West Nile virus Kunjin strain (WNVKUNV) | Dr. Philip Armstrong | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| ddhC | This study | N/A |
| Sucrose | Sigma-Aldrich | Cat#S0389 |
| Dithiothreitol (DTT) | American Bioanalytical | Cat#AB00490 |
| DPBS, no calcium, no magnesium | Thermo Fisher Scientific | Cat#14190144 |
| DMEM, high glucose | Thermo Fisher Scientific | Cat#11965092 |
| Fetal Bovine Serum | Gemini Bio | Cat#100–106 |
| Gibson Assembly Master Mix | New England Biolabs | Cat#E2611 |
| Q5 Hot Start High-Fidelity 2X Master Mix | New England Biolabs | Cat#M0494 |
| ZymoPURE II Plasmid Midiprep Kit | Zymo Research | Cat#D4201 |
| MEGAscript T7 Transcription Kit | Thermo Fisher Scientific | Cat#AMB13345 |
| ClaI | New England Biolabs | Cat#R0197S |
| EcoRI-HF | New England Biolabs | Cat# R3101S |
| Bovine Serum Albumin (BSA) | American Bioanalytical | Cat#AB01088 |
| TRIzol Reagent | Thermo Fisher Scientific | Cat#15596026 |
| Ethanol 200 proof | Decon Labs | Cat#2716 |
| GlycoBlue | Thermo Fisher Scientific | Cat#AM9515 |
| 4x Laemmli Sample Buffer | Bio-Rad | Cat#1610747 |
| Trans-Blot Turbo™ 5x Transfer Buffer | Bio-Rad | Cat#10026938 |
| Doxycycline | Sigma-Aldrich | Cat#D9891 |
| Universal Type I IFN | PBL Assay Science | Cat#11200–2 |
| Puromycin | Thermo Fisher Scientific | Cat#A1113803 |
| Glutamax | Thermo Fisher Scientific | Cat#35050061 |
| Saponin | Sigma-Aldrich | Cat#S7900 |
| Penicillin-Streptomycin | Thermo Fisher Scientific | Cat#15140122 |
| dialyzed fetal bovine serum | Thermo Fisher Scientific | Cat#A3382001 |
| [35S]-methionine/cysteine | PerkinElmer Life Science | Cat#NEG772014MC |
| Tris-HCl | American Bioanalytical | Cat#AB02005 |
| IGEPAL CA-630 | Sigma-Aldrich | Cat#I8896 |
| PhosSTOP | Sigma-Aldrich | Cat#PHOSS-RO |
| Protease inhibitor cocktail | Sigma-Aldrich | Cat#P8340 |
| RNaseOUT | Thermo Fisher Scientific | Cat#10777019 |
| Rapamycin | Cell Signaling Technology | Cat#9904 |
| 16% paraformaldehyde | Electron Microscopy Sciences | Cat#15710 |
| Goat serum | Thermo Fisher Scientific | Cat#16210064 |
| ProLong Gold Anti-Fade Reagent | Thermo Fisher Scientific | Cat#P36930 |
| Hoechst 33342 | Thermo Fisher Scientific | Cat#H3570 |
| O-Propargylpuromycin | Abcam | Cat#ab146664 |
| L-Ascorbic acid | Sigma-Aldrich | Cat#A4544 |
| Copper sulfate | Sigma-Aldrich | Cat#C1297 |
| Azide-fluor 488 | Sigma-Aldrich | Cat#760765 |
| Cycloheximide | Sigma-Aldrich | Cat#C4859 |
| Potassium acetate | Sigma-Aldrich | Cat#P5708 |
| Magnesium chloride | Sigma-Aldrich | Cat#M8266 |
| RNase A | Sigma-Aldrich | Cat#R6513 |
| Sodium chloride | American Bioanalytical | Cat#AB01915 |
| Ethylenediaminetetraacetic acid (EDTA) | Sigma-Aldrich | Cat#EDS |
| Acetonitrile | Sigma-Aldrich | Cat#34851 |
| Methanol | Sigma-Aldrich | Cat#34860 |
| Water | American Bioanalytical | Cat#AB02128 |
| Formic acid | Thermo Fisher Scientific | Cat#A117–50 |
| DMEM, high glucose, no glutamine, no methionine, no cystine | Thermo Fisher Scientific | Cat#21013024 |
| Thapsigargin | Sigma-Aldrich | Cat#T9033 |
| Sodium arsenite | Sigma-Aldrich | Cat#S7400 |
| Polyinosinic:polycytidylic acid [poly(I:C)] | Sigma-Aldrich | Cat#P9582 |
| Lipofectamine 2000 | Thermo Fisher Scientific | Cat#11668500 |
| OptiMEM | Thermo Fisher Scientific | Cat#11058021 |
| Integrated stress response inhibitor (ISRIB) | Sigma-Aldrich | Cat#SML0843 |
| 5-Ethynyluridine | Abcam | Cat#ab146642 |
| Tween-20 | Sigma-Aldrich | Cat#P7949 |
| Triton X-100 | American Bioanalytical | Cat#AB02025 |
| Critical commercial assays | ||
| Luna Universal One-Step RT-qPCR Kit | New England Biolabs | Cat#E3005 |
| Bradford protein assay | Bio-Rad | Cat#5000001 |
| Power SYBR Green (Thermo Fisher Scientific) | Thermo Fisher Scientific | Cat#4367659 |
| CellTrace CFSE Cell Proliferation Kit (Invitrogen) | Thermo Fisher Scientific | Cat#C34570 |
| Annexin V-FITC Apoptosis Detection Kit | Thermo Fisher Scientific | Cat#BMS500FI |
| SuperScript II Reverse Transcriptase | Thermo Fisher Scientific | Cat#18064014 |
| NEBNext rRNA Depletion Kit (NEB) | New England Biolabs | Cat#E6310 |
| TruSeq Stranded mRNA Library Prep | Illumina | Cat#20020594 |
| Deposited data | ||
| Raw and analyzed data | This paper | GEO: GSE142015 |
| Raw Western blot and microscopy images | This paper; Mendeley Data | DOI:10.17632/dyxgt2g7cm.2 |
| Experimental models: Cell lines | ||
| Human: HEK293T | ATCC | Cat#CRL-3216; RRID: CVCL_0063 |
| African green monkey: Vero | ATCC | Cat#CCL-81; RRID: CVCL_0059 |
| Human: HeLa | ATCC | Cat#CCL-2; RRID: CVCL_0030 |
| Hamster: BHK-21 | ATCC | Cat#CCL-10; RRID: CVCL_1915 |
| Mouse: iBMDM | This study | N/A |
| Mouse: iBMDM.VipKO | This study | N/A |
| Human: 293T.iVip | This study | N/A |
| Human: HEK293T-Cas9 | Dr. Jorge Galán Chang et al., 2016 |
N/A |
| Human: 293T.GCN2KO | This study | N/A |
| Human: 293T.ZAKKO | This study | N/A |
| Experimental models: Organisms | ||
| Mouse: B6 (C57BL/6J) | Jackson Laboratory | Cat#000664; RRID: IMSR_JAX:000664 |
| Mouse: Viperin KO (B6.129-Rsad2tm1Kchc/CresJ) | Seo et al., 2011 | RRID:IMSR_JAX:032321 |
| Oligonucleotides | ||
| All standard primers for qRT-PCR | This study | Table S1 |
| Recombinant DNA | ||
| pcDNA3.1 | Thermo Fisher Scientific | Cat#V79020 |
| pcDNA3.1-Venus | Hee and Cresswell, 2017 | N/A |
| pcDNA3.1-viperin | Seo et al., 2011 | N/A |
| pcDNA3.1-viperin (d1–42) | Seo et al., 2011 | N/A |
| pcDNA3.1-viperin (DCA) | Seo et al., 2011 | N/A |
| pcDNA3.1-GCN2 | This study | N/A |
| pcDNA3.1-GCN2(D858N) | This study | N/A |
| pCMV-VSV-G | Addgene | Cat#8454 |
| psPAX2 | Addgene | Cat#12260 |
| non-targeting control gRNA | Addgene | Cat#80263 |
| GCN2 gRNA 1 | Addgene | Cat#75875 |
| GCN2 gRNA 2 | Addgene | Cat#75876 |
| ZAK gRNA 1 | Addgene | Cat#75596 |
| ZAK gRNA 2 | Addgene | Cat#75597 |
| pTRIPZ-EV | This study | N/A |
| pTRIPZ-viperin | This study | N/A |
| pTRIPZ-viperin (DCA) | This study | N/A |
| pFLZIKV | Shan et al., 2016 | N/A |
| Software and algorithms | ||
| ImageJ | National Institutes of Health | RRID:SCR_003070 |
| STAR | Dobin et al., 2013 | RRID:SCR_004463 |
| Bedtools Multicov | Quinlan and Hall, 2010 | RRID:SCR_006646 |
| FlowJo | FlowJo LLC | RRID:SCR_008520 |
| Other | ||
| Mini-PROTEAN TGX Stain-Free™ Protein Gels | Bio-Rad | Cat#4568084 |
| SuperSep Phos-tag gel | Wako Chemicals | Cat#198–17981 |
| Immobilon-P PVDF membrane | Sigma-Aldrich | Cat#IPVH00010 |