| Antibodies |
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| Bacterial and virus strains |
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Escherichia coli Rosetta(DE3) |
Millipore Sigma |
Cat #69450–3 |
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| Biological samples |
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| Chemicals, peptides, and recombinant proteins |
| HEPES |
Fisher |
BP310 |
| TRIS |
Fisher |
BP154 |
| NaCl |
RPI |
S23030 |
| NaN3
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Fisher |
S227I |
| Dithiothreitol (DTT) |
Fisher |
R0861 |
| SYPRO orange dye |
Sigma |
S5692 |
| 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) |
Fisher |
AC117540010 |
| Urea |
Fisher |
U15 |
| Ammonium bicarbonate |
Fisher |
A643 |
| Ammonium acetate |
Fisher |
A637 |
| Ammonium sulfate |
Sigma |
S5636 |
| Morpheus screen |
Molecular Dimensions |
MD1–124 |
| Hampton index screen |
Hampton |
HR2–134 |
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| Critical commercial assays |
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| Deposited data |
| Crystal structure of γS-wt |
This paper |
PDB: 7N36 |
| Crystal structure of γS-3 |
This paper |
PDB: 7N37 |
| Crystal structure of γS-5 |
This paper |
PDB: 7N38 |
| Crystal structure of γS-7 |
This paper |
PDB: 7N39 |
| Crystal structure of γS-9A |
This paper |
PDB: 7N3A |
| Crystal structure of γS-9B |
This paper |
PDB: 7N3B |
| NMR structure of γS-wt |
(Kingsley et al., 2013) |
PDB: 2M3T |
| Crystal structure of γS dimer |
(Thorn et al., 2019) |
PDB: 6FD8 |
| Crystal structure of γS C-terminal domain |
(Purkiss et al., 2002) |
PDB: 1HA4 |
| Crystal structure of γS-9C |
(Norton-Baker et al., 2021) |
PDB: 7NJE |
| Experimental models: Cell lines |
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| Experimental models: Organisms/strains |
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| Oligonucleotides |
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| Recombinant DNA |
| pET28a(+) |
(Brubaker et al., 2011) |
N/A |
| Modifed pET28a(+) vectors |
This paper |
N/A |
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| Software and algorithms |
| XDS |
(Kabsch, 2010) |
N/A |
| Phaser |
(McCoy et al., 2007) |
N/A |
| Phenix version 1.18.2 |
(Liebschner et al., 2019) |
N/A |
| Coot version 0.9.4 |
(Emsley et al., 2010) |
N/A |
| PyMol version 2.4.1 |
Schrödinger |
https://pymol.org/2/
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| PROPKA |
(Olsson et al., 2011) |
propka.org
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| Prot pi |
Roland Josuran, Zurich University of Applied Sciences |
https://www.protpi.ch/Calculator/ProteinTool
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| Zetasizer |
Malvern |
N/A |
| OmniSEC |
Malvern |
N/A |
| Prism |
GraphPad Software |
http://www.graphpad.com/
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| Other |
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