Key resources table.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bacterial and virus strains
Escherichia coli Rosetta(DE3)	Millipore Sigma	Cat #69450–3
Biological samples
Chemicals, peptides, and recombinant proteins
HEPES	Fisher	BP310
TRIS	Fisher	BP154
NaCl	RPI	S23030
NaN3	Fisher	S227I
Dithiothreitol (DTT)	Fisher	R0861
SYPRO orange dye	Sigma	S5692
5,5-dithiobis(2-nitrobenzoic acid) (DTNB)	Fisher	AC117540010
Urea	Fisher	U15
Ammonium bicarbonate	Fisher	A643
Ammonium acetate	Fisher	A637
Ammonium sulfate	Sigma	S5636
Morpheus screen	Molecular Dimensions	MD1–124
Hampton index screen	Hampton	HR2–134
Critical commercial assays
Deposited data
Crystal structure of γS-wt	This paper	PDB: 7N36
Crystal structure of γS-3	This paper	PDB: 7N37
Crystal structure of γS-5	This paper	PDB: 7N38
Crystal structure of γS-7	This paper	PDB: 7N39
Crystal structure of γS-9A	This paper	PDB: 7N3A
Crystal structure of γS-9B	This paper	PDB: 7N3B
NMR structure of γS-wt	(Kingsley et al., 2013)	PDB: 2M3T
Crystal structure of γS dimer	(Thorn et al., 2019)	PDB: 6FD8
Crystal structure of γS C-terminal domain	(Purkiss et al., 2002)	PDB: 1HA4
Crystal structure of γS-9C	(Norton-Baker et al., 2021)	PDB: 7NJE
Experimental models: Cell lines
Experimental models: Organisms/strains
Oligonucleotides
Recombinant DNA
pET28a(+)	(Brubaker et al., 2011)	N/A
Modifed pET28a(+) vectors	This paper	N/A
Software and algorithms
XDS	(Kabsch, 2010)	N/A
Phaser	(McCoy et al., 2007)	N/A
Phenix version 1.18.2	(Liebschner et al., 2019)	N/A
Coot version 0.9.4	(Emsley et al., 2010)	N/A
PyMol version 2.4.1	Schrödinger	https://pymol.org/2/
PROPKA	(Olsson et al., 2011)	propka.org
Prot pi	Roland Josuran, Zurich University of Applied Sciences	https://www.protpi.ch/Calculator/ProteinTool
Zetasizer	Malvern	N/A
OmniSEC	Malvern	N/A
Prism	GraphPad Software	http://www.graphpad.com/
Other