Table 1.

Gene variants used to test Somatic SOP

Gene Symbol	KRAS	BRAF	PIK3CA	IDH1	EZH2	TERT	FLT3	PTEN	TP53	RB1
Transcript ID	NM_004985.5	NM_004333.6	NM_006218.4	NM_005896.4	NM_004456.4	NM_198253.3	NM_004119.3	NM_000314.6	NM_000546.5	NM_000321.2
Variant	p.Gly12Ala	p.Pro14Arg	p.Met1?	p.Phe32Val	c.-7-2A>C	c.-124C>T	p.Asp835Tyr	c.-9C>G	p.Pro47Ser	p.Ile124ArgfsTer6
	p.Gln61Lys	p.Ala31Gly	p.Ile391Met	c.123-4C>T	p.Asp136Val	c.-146C>T	p.Asp835Glu	p.His123Arg	p.Val73Met	p.Ser443Ter
	p.Ser65Ile	p.Pro403LeufsTer8	p.Glu418Lys	p.Val71Ile	p.Asp233Gly	c.-124C>A	p.Asp835His	p.Arg130Gln	p.Cys124Ter	c.607+1G>A
	p.Arg164Gln	p.Asp445His	p.Glu453Lys	p.Arg132His	p.Val626Met	c.-332C>T	p.Asp835Val	p.Thr131Ile	p.Cys135Gly	c.2211+5G>T
		p.Asn486_Pro490del	p.Glu545Ala	p.Arg132Cys	p.Arg658Thr	c.-245T>C	p.Asp835Ile	p.Leu182Ser	p.Val173Met	c.1216-29A>G
		p.Gly469Ala	p.Lys733Arg	p.Arg132Ser	p.Ala682Gly	p.Arg230Ter	p.Asn676Lys	p.Leu193=	p.Glu180Lys	p.Gln689His
		p.Gly469Glu	p.His1047Gln	p.Val178Ile	p.Ala682Val	p.Trp581Ter	p.Tyr842Cys	p.Pro204Ala	p.Glu298Lys	p.Ser567Leu
		p.Asp594Asn	p.His1047Arg	p.Asp220Gly	p.Arg684Cys		p.Phe691Leu	c.634+5G>C	p.Gly334Arg	p.Trp563Leu
		p.Val600Lys	p.His1065Tyr	p.Glu262Lys	p.Arg690His		p.Tyr693Cys	p.Lys322Ter	p.Ala347Asp	p.Arg661Trp
		p.Lys601Glu	p.Asn1068LysfsTer5		p.Ser695Leu		p.Tyr693Asn	p.Pro354Leu	p.His365Tyr	p.Glu554Lys
					p.Tyr731Phe					p.Tyr606Cys
					p.Tyr731His
					p.Tyr646Phe

“p.“ denotes the variant at the protein level, “c.“ denotes the variant at the cDNA level. Note, the first 5 TERT variants are in promoter region and the negative number indicates the number of base pairs upstream of the ATG translation start site.