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. Author manuscript; available in PMC: 2022 Nov 2.
Published in final edited form as: Nat Neurosci. 2022 May 2;25(5):567–576. doi: 10.1038/s41593-022-01060-2

Extended Data Fig. 5. Analysis of calvarial leukocytes during meningitis.

Extended Data Fig. 5

a, Experimental outline of calvarial leukocyte analysis. b, Flow cytometry gating. c-f, Quantitation of calvarial leukocytes 6 hours after intracisternal S. pneumococci injection shows neutrophils, monocyte subsets and total lymphocytes (n=5 mice per group). g-j, Quantitation of calvarial leukocytes 12 hours after S. pneumococci injection including neutrophils (g), Ly6Chi monocytes (h), Ly6Clo monocytes (i) and total lymphocytes (j) (n=9 sham and 8 meningitis). k-n, Quantitation of calvarial leukocytes 24 hours after S. pneumococci injection including neutrophils (k), Ly6Chi monocytes (l), Ly6Clo monocytes (m) and total lymphocytes (n) (n=6 mice per group). (P values represent unpaired, two-tailed t-tests, data are mean values ± SD).