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. Author manuscript; available in PMC: 2022 Nov 2.
Published in final edited form as: Nat Neurosci. 2022 May 2;25(5):567–576. doi: 10.1038/s41593-022-01060-2

Extended Data Fig. 8. Myd88-related sensing in the skull marrow.

Extended Data Fig. 8

a, Experimental groups include wild type and Myd88−/− mice. The skull marrow was assessed by flow cytometric staining for lineage markers Sca-1 and c-kit. b, Flow cytometry plots and c, quantitation of LSK % as a total of all live lineage negative single cells in the calvarial marrow of steady-state Myd88−/− or wild-type C57/Bl6 mice (n=9 sham and 6 meningitis mice, P value represents an unpaired, two-tailed t-test, data are mean values ± SD). d, Experimental outline. Non-irradiated wild type recipient mice received a mix of 40,000 LSK from wild type donors (labeled with the membrane dye DiD) and from Myd88−/− donors (labeled with Dil). e, Flow cytometry gating and f, analysis of the skull bone marrow 3 days later showed a similar seeding of LSK irrespective of phenotype (n=4 mice per group, unpaired, two-tailed t-test).g, Experimental outline of calvarial progenitor analysis in Myd88−/− mice with and without meningitis. h, Flow cytometry gating. i, Quantitation of calvarial BrdU+ CMP 12 hours after intracisternal sham or S. pneumococci injection. (n=11 sham and 12 meningitis mice per group, unpaired two-tailed t-test, data are mean values ± SD). j, Quantitation of calvarial BrdU+ LSK 24 hours after intracisternal sham or S. pneumococci injection. (n=6 mice group, unpaired two-tailed t-test, data are mean values ± SD).