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. Author manuscript; available in PMC: 2022 Nov 2.
Published in final edited form as: Nat Neurosci. 2022 May 2;25(5):567–576. doi: 10.1038/s41593-022-01060-2

Extended Data Fig. 1. CSF tracer outflow in occipital, parietal and frontal skull bones.

Extended Data Fig. 1

a, Experimental outline. Ex-vivo z-stack (54 μm stack at 1 μm/step) of occipital, parietal and frontal skull cortex after IC and IV injection of fluorescently labeled dextran. Bone is visualized by second harmonic generation around channels b, Imaging of CSF tracer outflow through channels in different skull bones, assessed in n=2 mice. Bar graphs depict the proportion of skull channels that were positive for CSF tracer.