Skip to main content
. Author manuscript; available in PMC: 2023 May 5.
Published in final edited form as: Mol Cell. 2022 Mar 30;82(9):1768–1777.e3. doi: 10.1016/j.molcel.2022.03.008

Table 2.

Comparison of agarose-based electrophoresis systems on circular RNA migration.

Agarose Gel Advantages Disadvantages
E-Gel EX

  • Distinguish between circular RNA and linear RNA of equivalent lengths

  • Fast/convenient

  • Expensive

  • May show variability between lots (see Figure S1).
E-Gel (non-EX)

  • Fast/convenient

  • Circular RNA overlaps with linear RNA of equivalent lengths
Self cast agarose gels

  • Migrates near expected size

  • Ability to control running buffer conditions and voltage settings

  • Optimal for Northern blots

  • Time consuming

  • Circular RNA overlaps with linear RNA of equivalent lengths