Extended Data Fig. 6. Fecal microbiome transfer in high PA humanized mice results in a compositional changes.

a, Microbiome profiles of high PA humanized mice receiving either a control or an FMT with healthy microbiota (n=9 mice/group) were compared at the phyla, class, and family levels. At both phyla and class levels of taxonomy, no significant differences were observed between the two groups of mice. However, at the family level, 9 differential taxa were observed. With increased abundance of Prevotellaceae, Eubacteriaceae, Enterobacteriaceae, Bacteroidaceae, and Clostridiaceae in controls while Odoribacteraceae, Rikenellaceae, Barnesiellaceae and Sutterellaceae were more abundant in mice receiving FMT (FDR<0.1).b, Correlation scatterplots for differentially abundant taxa that negatively correlate with PA post-FMT treatment. After FMT, three bacterial species were found to negatively correlate with fecal PA, all at a q< 0.1. Taxa identified were A. putredinis, Barnesiella intestinihominis, and A. obesi. All of these taxa had strong negative correlations with PA status in mice post-FMT (0.6 ≤ |r| ≤ 0.79). Correlation coefficients (r) and q-values are generated from comparisons within FMT and control animals to assess the relationship between PA and differentially abundant taxa (n=9 mice/group). Grey area shows 95% confidence level for linear smooths.