Freeze–thaw pretreatment conditions.
Samples | Temperature (°C) | Time (h) |
---|---|---|
A1 | −4 °C | 12 |
A2 | 24 | |
A3 | 48 | |
B1 | −20 °C | 12 |
B2 | 24 | |
B3 | 48 | |
CK | — | — |
Samples | Temperature (°C) | Time (h) |
---|---|---|
A1 | −4 °C | 12 |
A2 | 24 | |
A3 | 48 | |
B1 | −20 °C | 12 |
B2 | 24 | |
B3 | 48 | |
CK | — | — |