Fig. 2. Readout of the 96-well plate o-xylylenediamine assay for the novel TA. Clone A: E. coli transformed with pET-TA but not fed with benzaldehyde; clone B: E. coli transformed with pET-TA; clone C: cell lysate of E. coli transformed with pET-TA; clone D: E. coli transformed with S2A24 fosmid; clone E: cell lysate of E. coli transformed with S2A24 fosmid. With the exception of clone A, which was not provided with any benzaldehyde, all wells are fed with 5 mM benzaldehyde and 2 mM PLP. The volume of each reaction mixture is 200 μL and the plate is maintained at 30 °C and pH 7.5. For reaction conditions 1–3, 5 mM of o-xylylenediamine was supplied to the well. For reaction conditions 4–6, 25 mM of o-xylylenediamine was supplied to the well. The raw image of the plate is included in the ESI.†.