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. 2022 Apr 20;12(8):e4392. doi: 10.21769/BioProtoc.4392

Figure 6. Expected results for the detection of HBV RNA.

Figure 6.

A) PCR Curve of the different sample conditions with HBV specific (left) and B2M specific (right) primers and probes. B) Observed Ct values and average Ct values, as well as the HBV RNA fold change calculations for the different samples compared to the organoids infected with heat-inactivated HBV. C) Bar graph of the HBV RNA fold changes present in the different samples. The y-axis represents the fold changes, and the x-axis represents the different sample conditions.