Component	Final concentration
2× LightCycler480 Probes Master (Roche)		1× (12.5 µL)
Forward Primer (100 µM)	0.5 µM
Reverse Primer (100 µM)	0.5 µM
Probe (50 µM)	0.1 µM
DNA template	4 µL
Nuclease free water	x µL
Total reaction volume	25 µL