Component	Final concentration
2× LightCycler480 Probes Master (Roche)	1× (10 µL)
Forward Primer (100 µM)	1 µM
Reverse Primer (100 µM)	1 µM
Probe (50 µM)	0.2 µM
DMSO	4%
cccDNA template	4.2 µL
Nuclease free water	x µL
Total reaction volume	20 µL