Component	Final concentration
10× PCR Buffer	1× (2.5 µL)
50 mM MgCl2	1.75 mM
10 mM dNTP mix	400 µM
Forward Primer (100 µM)	0.5 µM
Reverse Primer (100 µM)	0.5 µM
Probe (50 µM)	0.15 µM
Platinum Taq DNA Polymerase	0.04units/µL
cDNA template (diluted 1:2.5 or 1:5 in water, based on the starting amount of RNA used for cDNA synthesis)	4 µL
Nuclease free water	x µL
Total reaction volume	25 µL