Fig. 2. Effect of cell density scatter among analyzed image fields (replicates) on nanoparticle association results as determined by confocal imaging followed by 2D image analysis for fluorescence intensity of the nanoparticle signal using ImageJ. A schematic depicting the cell density scatter among both groups; wide-scattered and tight-scattered cell densities (A) and a bar chart showing the actual cell densities of representative experiments (B) are represented. (C) Different significant levels were observed for quantum dot and liposome association with HUVEC reference to control experiments with cells not exposed to nanoparticles. Data points represent mean ± standard deviation (n ≥ 3). * denotes significant difference from control (p < 0.05) as determined by one way ANOVA and post hoc Tukey HSD test.