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. 2022 Apr 25;4:881347. doi: 10.3389/ftox.2022.881347

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Copyright © 2022 Blake, Rickard and Fenton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Examples of chemical structures and corresponding phase contrast live cell images obtained from JEG-3 cells after 24 h exposure to PFAS with most pronounced effects on cellular proliferation. (A) Structure of perfluorooctanamide (423-54-1; EC50 ± SE: 114.0 ± 35.6 µM); (B) Live cell image of cells exposed to 150 µM 423-54-1; (C) Structure of 6:2 fluorotelomer phosphate diester (57677-95-9; EC50 ± SE: 141.6 ± 39.4 µM); (D) Image of cells exposed to 150 µM 57677-95-9; (E) Structure of perfluorooctane sulfonamide (754-91-6; EC50 ± SE: 159.8 ± 90.0 µM); (F) Image of cells exposed to 150 µM 754-91-6. No overt cell death is apparent in (B), (D), or (F).