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Fig. 1. The proposed “OFF–ON” detection principle using Qdot-aptamer conjugates and 3D nanoporous photonic crystal. (a) Loading the prepared quenched Qdot-aptamer conjugates (i.e., “OFF” state) for influenza A (H1N1) virus detection in aerosol spray. (b) Capturing airborne influenza A (H1N1) virus by aerosol spray and recovering Qdot signals by releasing G-DNA and preferred aptamer-virus binding (i.e., “ON” state). (c) Visualization of virus detection by enhanced Qdot signal owing to the emission light guide in 3D PC.