Comparison of the assay time and LOD in the proposed influenza H1N1 assay with other reported techniques.

Reference	Method	Assay time	LOD
Su et al.,20 2012	Surface plasmon resonance	20 min	1.5a pg mL−1
Feng et al.,21 2015	PCR-based SELEX	80 min	50b pg mL−1
Tseng et al.,22 2016	Fluorescence	9 steps, total 30 min	8c ng mL−1
This work	Fluorescence & light guiding	40 min	138 pg mL−1

^a30 PFU mL⁻¹ = ∼3 × 10³ particles per mL = ∼1.5 pg mL⁻¹.

^b1 × 10² TCID50/100 μL = ∼1 × 10⁴ particles per mL = ∼50 pg mL⁻¹.

^c0.032 HAU/20 μL = ∼6.4 × 10⁶ particles per mL = ∼8 ng mL⁻¹.²³