Fig. 2. Cellular uptake studies of DOX formulations. (A) CLSM images of 4T1 cells incubated with DOX, DOX-Lip and ITZ/DOX-PLip for 4 hours at 37 °C, respectively. Red represents the fluorescence of DOX and blue represents the fluorescence of DAPI, scale bar represents 10 μm. (B) FCM curves of 4T1 cells and (C) quantification of intracellular DOX after incubation with DOX, DOX-Lip and ITZ/DOX-PLip for 4 hours at 37 °C, respectively. Each bar represents mean fluorescence intensity ± standard deviation (n = 3). **p < 0.01.
