Fig. 5. In vivo antitumor efficacy of free drugs and liposomes with different dosages in 4T1 cells tumor-bearing mice (n = 5). Mice were injected with normal saline as control, DOX (5 mg kg⁻¹, every three days), ITZ (2.5 mg kg⁻¹, every three days), and ITZ/DOX-PLip (DOX 5 mg kg⁻¹, ITZ 2.5 mg kg⁻¹, every three days). Throughout the study, mice were weighed and tumors were measured with calipers every two days. (A) The changes in tumor growth and (B) body weight were measured. At the end of the trial, tumor tissues were removed and weighted, and (C) the tumor growth inhibition (TGI%) were calculated. The results are shown as the mean ± standard deviation (n = 5). *p < 0.05, **p < 0.01.