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. 2022 Apr 25;13:897174. doi: 10.3389/fphys.2022.897174

FIGURE 5.

FIGURE 5

Interspecies comparisons of relative OCRs in the permeabilized thoraces of the three insect species (A. mellifera carnica, D. melanogaster, and L. decemlineata). (A) CI-OXPHOS, (B) CI + ProDH-OXPHOS, (C) CI + ProDH + CII-OXPHOS, and (D) CI + ProDH + CII + mtG3PDH-OXPHOS. Relative OCRs are reported as mean ± s.e.m and were obtained by dividing each OCR by the corresponding complex IV maximal OCR for 6 (n = 6–10), 12 (n = 6–10), 18 (n = 5–10), 24 (n = 5–10), 30 (n = 6–10), 36 (n = 6–10), 42 (n = 6–10) and 45°C (n = 5–9). Relative OCRs were compared between species for each assay temperature using a one-way ANOVA followed by a HSD (Honest Significant Difference) Tukey’s test. Dissimilar letters indicate significant differences between species at each assay temperature. For CI + ProDH + CII-OXPHOS at 30°C, one-way ANOVA conditions were not met, and a non-parametric test (Kruskall-Wallis) followed by a Dunn’s test were performed.