Fig. 5. P53, bcl-2, caspase-3, caspase-9, and BAX expression in the cardiac tissues of all groups after 8 weeks with different treatments. (A) Immunoblotting for p53 and bcl-2 in cardiac tissues. (B) Bar graph showing quantification of p53 and bcl-2 protein expression. Data are given as the mean ± SEM; n = 3 in each group. *p < 0.05 vs. DOX group. (C) Representative immunohistochemistry staining for caspase-3, caspase-9, and BAX expression in cardiac tissues. Scale bar = 200 μm. Arrows indicate positively stained cells.