Figure EV1. Influence of FMRP protein on human DGKk protein expression and on mouse DGKk mRNA level.

1. Immunoblots and quantification of lysates from Hela cells transfected with plasmid pCI‐hDGKk encoding human DGKk and pre‐transfected 24 h before with siRNA control (siC) or against FMRP (siFMR1). GAPDH was used as a loading control. For quantification, DGKk and FMRP signals were normalized against GAPDH signal and presented relative to the signal for siC‐treated cells. Each point represents data from an individual culture, and all values are shown as mean ± SEM ***P < 0.001, calculated by unpaired Student t‐test.
2. Quantification of mouse DGKk mRNA by qRT‐PCR in RNA extracts of Cos‐1 cells transfected with plasmid pCI‐mDGKκ‐HA and pre‐transfected with siRNA control (siC) or siRNA against FMRP (siFMR1) or mock transfected (NT). Each point represents data from an individual culture, and all values are means of fold change ± SEM, determined using ∆∆Ct method with Actb as normalizer, n = 3 biological replicates. P values were calculated using one‐way ANOVA with Tukey's multiple comparison test. ***P < 0.001; ns, P > 0.05.

Source data are available online for this figure.