Figure EV3. ∆N‐DGKk expression in brain with AAV vectors is stable over time and normalizes abnormal phosphatidic acid level in Fmr1‐KO cortex.
- Representative coronal brain sections processed for detection of ∆N‐DGKk (HA) at 8 and 12 weeks post‐injections using immunohistochemistry on Fmr1‐KO mice treated with indicated treatment, counterstained with eosin hematoxylin. Three regions, a, b, c, are shown with their corresponding position on brain sagittal map. Scale bar 2 mm. Image of brain 8W b is a reuse of Fig 3D Rh10.
- Immunoblots and quantification of ∆N‐DGKk protein in Fmr1‐WT brain lysates from cortex (c), hippocampus (h), and rest (r) areas as in Fig 3C. GAPDH was used as a loading control. AU, arbitrary units.
- Measure of total phosphatidic acid (PA) level by mass spectrometry in hippocampus and rest of brain of WT mice treated with saline solution (WT) and Fmr1‐KO mice treated with saline (Fmr1‐S), AAVPHP.eB‐∆N‐DGKk (Fmr1‐PHP.eB), AAVRh10‐∆N‐DGKk (Fmr1‐Rh10) 8 weeks after injections. Data are expressed as mol % of total lipids and analyzed using one‐way ANOVA and Tukey’s multiple comparisons test, n = 8 individual animals, except for WT and Fmr1‐S n = 11 and represented as median with interquartile range with minimum and maximum values.
- Total diacylglycerol (DAG) level in cortex measured as in C).
Source data are available online for this figure.