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. 2022 Apr 25;9:889963. doi: 10.3389/fcvm.2022.889963

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Copyright © 2022 Varasteh, Braeuer, Mohanta, Steinsiek, Habenicht, Omidvari, Topping, Rischpler, Weber, Sager, Raes, Hernot and Schwaiger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative in vivo imaging of ⁶⁸Ga-NOTA-anti-MMR Nb uptake in a longitudinal study. (A) Static PET/CT matched axial, coronal and sagittal slices in the same mouse subjected to coronary ligation and scanned 1 h after injection of ⁶⁸Ga-NOTA-anti-MMR Nb (2, 7, and 14 days after MI) and ¹⁸F-FDG (7 days after MI). In order to validate the results obtained by in vivo PET/CT imaging (in n = 6 mice) and to confirm the cardiac origin of the in vivo signal, hearts from two MI-induced mice were also scanned ex vivo. (B) MR only and (C) PET/MRI of the heart excised from a mouse, 7 days after coronary ligation, confirming augmented ⁶⁸Ga-NOTA-anti-MMR Nb uptake in the area close to the ligation site.