KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-substance P monoclonal antibody | Millipore | Cat# MAB356, clone NC1; RRID: AB_94639 |
| Goat anti-rat IgG-FITC antibody | Invitrogen | Cat# 31629; RRID: AB_228240 |
| Purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™) | BD Biosciences | Cat# 553142; RRID: AB_394656 |
| FITC anti-mouse CD64 (FcγRI) Antibody | BioLegend | Cat# 139316; RRID: AB_2566556, clone X54-5/7.1 |
| PE anti-mouse CD170 (Siglec-F) Antibody | BD Biosciences | Cat# 552126; RRID: AB_394340, clone E50-2440 |
| PerCP-Cy5.5 anti-mouse CD11c Antibody | BioLegend | Cat# 117326; RRID: AB_2129643, clone N418 |
| PE/Cyanine7 anti-mouse Ly-6G Antibody | BioLegend | Cat# 127618; RRID: AB_1877261, clone 1A8 |
| APC anti-mouse I-A/I-E Antibody (MHC-II) | BioLegend | Cat# 107614; RRID: AB_313329, clone M5/114.15.2 |
| APC/Cyanine7 anti-mouse Ly-6C Antibody | BioLegend | Cat# 128026; RRID: AB_10640120, clone HK1.4 |
| Brilliant Violet 421™ anti-mouse CD45.2 Antibody | BioLegend | Cat# 109832; RRID: AB_2565511, clone 104 |
| PE anti-mouse/human CD11b Antibody | BioLegend | Cat# 101208; RRID: AB_312790, clone M1/70 |
| FITC anti-mouse CD8a Antibody | BioLegend | Cat# 100706; RRID: AB_312745, clone 53-6.7 |
| APC anti-mouse FcεRIα Antibody | BioLegend | Cat# 134316, RRID; AB_10640121, clone MAR-1 |
| PerCP/Cyanine5.5 anti-mouse CD4 Antibody | BioLegend | Cat# 100540, RRID:; AB_893326, clone RM4-5 |
| PE-Cy™7 rat anti-mouse CD19 | BD Biosciences | Cat# 552854; RRID: AB_394495, clone 1D3 |
| PerCP-Cy5.5 anti-mouse CD117 (c-Kit) Antibody | BioLegend | Cat# 105825; RRID: AB_1626278, clone 2B8 |
| APC/Cyanine7 anti-mouse CD3 Antibody | BioLegend | Cat# 100222; RRID: AB_2057374, clone 17A2 |
| FITC anti-mouse/human CD11b Antibody | BioLegend | Cat# 101206; RRID: AB_312789, clone M1/70 |
| Mouse IgG antibody 4G2 | Gentry et al. (1982) Provided by Dr. Steve Whitehead, NIAID, NIH. | N/A |
| Peroxidase-labeled goat anti-mouse IgG (used for virus titration) | SeraCare | Cat# 5450-0011; RRID: AB_2687537 |
| Bacterial and virus strains | ||
| 2015 Fortaleza Zika virus strain | Calvet et al. (2016). | N/A |
| Provided by Dr. Steve Whitehead, NIAID, NIH. | ||
| Biological samples | ||
| Mouse third-order mesenteric arteries | This paper | N/A |
| Bovine whole blood in Acid Citrate Dextrose (ACD) | Lampire Biological Laboratories | Cat# 7200801 |
| Chemicals, peptides, and recombinant proteins | ||
| Pilocarpine hydrochloride | Sigma-Aldrich | Cat# P6503 |
| Sialokinin I peptide: NH2-NTGDKFYGLM-Amide | Atlantic Peptides | N/A |
| Substance P peptide: NH2-RPKPQQFFGLM-Amide | Atlantic Peptides | N/A |
| 4,5-Diaminofluorescein (DAF-2) | Cayman Chemical | Cat# 85160 |
| L-NG-Nitro arginine methyl ester (L-NAME) | Sigma-Aldrich | Cat# N5751 |
| Krebs-HEPES buffer | Boston BioProducts, Inc. | Cat # BSS-265 |
| LC Green Plus+ | Biofire Defense | Cat# BCHM-ASY-0005 |
| Pfx polymerase | Thermo Fisher Scientific | Cat# 11708039 |
| Paraformaldehyde | Fisher Scientific | Cat# 50-980-487 |
| Bovine Serum Albumin | Sigma-Aldrich | Cat# A9576 |
| Triton X-100 | EMD Millipore, Fisher Scientific | Cat# M1122980101 |
| Phalloidin Alexa Fluor 647 fluorophore | Thermo Fisher Scientific | Cat# A22287 |
| Vectashield with DAPI | Fisher Scientific | Cat# NC1695563 |
| Aprepitant | Sigma-Aldrich | Cat# 170729-80-3 |
| Dimethyl sulfoxide (DMSO) | Fisher Scientific | Cat# BP231-100 |
| ATP | Sigma-Aldrich | Cat# 34369-07-8 |
| Drabkin’s reagent | Sigma-Aldrich | Cat# D5941 |
| ACK lysis buffer | Lonza | Cat# 10548E |
| Minimum Essential Medium | Gibco, Thermo Fisher Scientific | Cat# 15188319 |
| Sodium pyruvate | Gibco Thermo Fisher Scientific | Cat# 12539059 |
| L-glutamine | Gibco, Thermo Fisher Scientific | Cat# 25030149 |
| Non-essential amino acids | Gibco, Thermo Fisher Scientific | Cat# 11-140-050 |
| Methylcellulose | Sigma-Aldrich | Cat# M0512 |
| OptiMEM medium | Gibco, Thermo Fisher Scientific | Cat# 31985062 |
| Penicillin—Streptomycin—Amphotericin B | Gibco, Thermo Fisher Scientific | Cat# 15240096 |
| TrueBlue™ Peroxidase Substrate | Seracare | Cat# 5510-0030 |
| RPMI 1640 media | Sigma-Aldrich | Cat# R8758 |
| Phorbol-12-myristate-13-acetate (PMA) | Sigma-Aldrich | Cat# P8139 |
| Nigericin | Sigma-Aldrich | Cat# N7143 |
| Vascular Cell Basal Medium | ATCC | Cat# PCS-100-030 |
| 70-kDa FITC-dextran | Sigma-Aldrich | Cat# FD70S |
| Critical commercial assays | ||
| DNA purification kit | Macherey-Nagel | Cat# 740609.50 |
| MEGAscript™ T7 Transcription Kit | Thermo Fisher Scientific | Cat# AMB13345 |
| MEGAclear™ Transcription Clean-Up Kit | Thermo Fisher Scientific | Cat# AM1908 |
| Phire Animal Tissue Direct PCR Kit | Fisher Scientific | Cat# F140WH |
| Multi Tissue Dissociation kit-1 | Miltenyi Biotec | Cat# 130-110-201 |
| LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit | Thermo Fisher Scientific | Cat# L34959 |
| Human ProcartaPlex mix & match 12-plex magnetic Luminex Assay kit | Thermo Fisher Scientific | Cat# PPX-12-MXTZ962 |
| Caspase-Glo 1 Inflammasome Assay | Promega | Cat# G9951 |
| Microvascular Endothelial Cell Growth Kit-BBE | ATCC | Cat# PCS-100-040 |
| Deposited data | ||
| Mass Spectrometry data from salivary glands of WT and KO mosquitoes | This paper | Table S1 |
| Mass Spectrometry data from saliva of WT and KO mosquitoes | This paper | Table S2 |
| Experimental models: Cell lines | ||
| C6/36 cells | ATCC | Cat# CRL-1660 |
| THP-1 cells | ATCC | Cat# TIB-202 |
| Vero cells | ATCC | Cat# CCL-81 |
| Human Primary Dermal Microvascular Endothelial Cells (HDMVECn) | ATCC | Cat# PCS-110-010 |
| Experimental models: Organisms/strains | ||
| Aedes aegypti Liverpool strain wild type | Virginia Tech, VA, USA | N/A |
| Aedes aegypti Liverpool strain sialoKi5 KO homozygous | This paper | N/A |
| Aedes aegypti Liverpool strain sialoKΔ8 KO homozygous | This paper | N/A |
| C57BL/6NCrl mice | Charles River Laboratories | Cat# 027 |
| BALB/cAnNCrl mice | Charles River Laboratories | Cat# 028 |
| Oligonucleotides | ||
| Synthetic guide RNAs | This paper | See Table S3 |
| SIAL-F: 5′-AGGATTTAATCTAACGTTGT-3′ | Eurofins Genomics | N/A |
| SIAL-R: 5′-TGTAGCTGTTGGAAGAGAA-3′ | Eurofins Genomics | N/A |
| Cas9 mRNA | Basu et al. (2015) | N/A |
| Software and algorithms | ||
| Gen 5 software | Biotek Instruments | https://www.biotek.com/products/software-robotics-software/gen5-microplate-reader-and-imager-software/ |
| GraphPad Prism v7 | GraphPad Software | https://www.graphpad.com/ |
| Lightscanner Call-IT 2.0 software | Basu et al. (2016) | https://lightscanner.software.informer.com/2.0/ |
| Kojin et al. (2021) | ||
| BioRender software | BioRender | https://biorender.com/ |
| ImageJ software v1.52a | Schneider et al. (2012) | https://imagej.nih.gov/ij/ |
| EdgeR | Kojin et al. (2021); Robinson et al. (2010) | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| Leica Application Suite X software | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| PIMSoft program v1.5 | PeriMed | https://pimsoft.software.informer.com/1.5/ |
| FlowJo software v10.6.1 | TreeStar Technologies | https://www.flowjo.com/ |