Fig. 5. Hesperetin and 5-FU synergistically affected apoptosis-related protein expression. (A) Bax and Bcl-2 expression levels were detected by western blot. The Bcl-2/Bax ratio was calculated (a, control; b, 5-FU at 20 μM; c, hesperetin at 200 μM; d, 20 μM 5-FU + 200 μM hesperetin. a, b, c and d is same in B). (B) Procaspase-9, cleaved caspase-9, procaspase-3, and cleaved caspase-3 levels were assessed (1, cleaved caspase-9/procaspase-9; 2, cleaved caspase-3/procaspase-3). Data are presented as means ± SEM (n = 3). *p < 0.05 versus control. ^#p < 0.05 versus combination.