FIG. 2.

Immunoelectron microscopy of P. aeruginosa PAO1 incubated with CAP18 (50 μg/ml) and detected with goat antiserum to CAP18 and PG-CG. PAO1 incubated with CAP18 (50 μg/ml) and preimmune goat serum and PG-CG did not contain any label (A). At time zero, PAO1 was morphologically normal and there was PG-CG labeling on the outer membrane, in the periplasm, and already throughout the cytoplasm (B). Extensive amounts of labeled outer membrane material were already sloughing from the bacterial cell (arrow). At 0.5 h, the amounts of extracellular debris increased and the integrity of the inner and outer membranes (arrow) began to deteriorate (C and D). At 4 h, many cells showed evidence of membrane damage (arrow) and extensive labeling throughout the cytoplasm (E). At 8 h, most bacterial cells were lysing (F) and the outer envelope was missing (arrow).