TABLE 2.
Antimicrobial activities of SMAP28, SMAP29, and their congenersa
| SMAP peptide |
Mannheimia haemolytica serovar:
|
Pasteurella trehalosi serovar 4
|
Salmonella enterica subsp. arizonae
|
Pasteurella multocida
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1
|
2
|
6
|
||||||||||
| MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | |
| 28 | 0.9 ± 0.3 | 0.5 ± 0.1 | 1.7 ± 0.4 | 0.6 ± 0.0 | 0.6 ± 0.0 | 0.6 ± 0.0 | 1.3 ± 0.0 | 0.8 ± 0.2 | 1.3 ± 0.0 | 2.1 ± 0.4 | 0.3 ± 0.0 | 0.3 ± 0.0 |
| 29 | 0.6 ± 0.0 | 0.6 ± 0.0 | 0.8 ± 0.2 | 3.8 ± 3.1 | 1.3 ± 0.0 | 1.3 ± 0.0 | 2.5 ± 0.0 | 3.3 ± 0.8 | 0.8 ± 0.2 | 1.5 ± 0.6 | 0.6 ± 0.0 | 0.6 ± 0.0 |
| 29-18 | >20.0 | >20.0 | 0.9 ± 0.4 | 0.7 ± 0.3 | >20.0 | >20.0 | ≥20.0 | ≥20.0 | >20.0 | >20.0 | ≥20.0 | ≥20.0 |
| 29-20 | >20.0 | >20.0 | 10.8 ± 5.1 | 2.1 ± 0.4 | ≥20.0 | ≥20.0 | 20.0 ± 0.0 | 11.7 ± 4.4 | >20.0 | >20.0 | >20.0 | >20.0 |
| 29-21 | >20.0 | >20.0 | 2.1 ± 0.4 | 5.0 ± 2.5 | >20.0 | >20.0 | ≥20.0 | 10.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 |
| 29-18AA | 10.0 ± 0.0 | ≥20.0 | 8.3 ± 1.7 | 8.3 ± 1.7 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | ≥20.0 | ≥20.0 |
|
Klebsiella pneumoniae
|
P. aeuruginosa PAO1
|
Corynebacterium pseudotuberculosis ATCC 19410
|
Staphylococcus avreus
|
A. fumigatus
|
Hemolytic activity (%) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | |
| 0.4 ± 0.2 | 0.4 ± 0.2 | 0.6 ± 0.0 | 1.7 ± 0.4 | 20.0 ± 0.0 | ≥20.0 | 1.3 ± 0.0 | 1.0 ± 0.2 | 5.0 ± 0.0 | 11.7 ± 4.4 | ND |
| 0.6 ± 0.0 | 0.6 ± 0.0 | 0.8 ± 0.2 | 0.8 ± 0.2 | >20.0 | >20.0 | 2.5 ± 0.0 | 2.5 ± 0.0 | ND | ND | 7.9 ± 0.01 |
| ≥20.0 | >20.0 | 1.3 ± 0.0 | 1.3 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | >40.0 | >40.0 | 3.6 ± 0.01 |
| 20.0 ± 0.0 | 20.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >40.0 | >40.0 | 4.0 ± 0.01 |
| 8.3 ± 1.7 | 8.3 ± 1.7 | 20.0 ± 0.0 | ≥20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >40.0 | >40.0 | 3.8 ± 0.01 |
| >20.0 | >20.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | >40.0 | >40.0 | ND |
a
Amino acid sequences are shown in Table 1. MICs and MBCs are shown in micrograms per milliliter as means ± standard errors of the means of three replications. ND, not determined.