TABLE 3.
Antimicrobial activities of ovispirin and congenersa
| Ovispirin peptide |
Mannheimia haemolytica serovar:
|
Pasteurella trehalosi serovar 4
|
Salmonella enterica subsp. arizonae
|
Pasteurella multocida
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1
|
2
|
6
|
||||||||||
| MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | |
| OV | 3.8 ± 1.0 | ND | ND | ND | 2.1 ± 0.4 | 2.1 ± 0.4 | 10.0 ± 0.0 | 10.0 ± 0.00 | 6.7 ± 1.7 | 8.3 ± 1.7 | 8.3 ± 1.7 | 8.3 ± 1.7 |
| OV-1 | 1.5 ± 0.6 | 0.8 ± 0.2 | 0.6 ± 0.0 | 0.5 ± 0.1 | 0.2 ± 0.0 | 0.2 ± 0.0 | 2.3 ± 1.4 | 1.0 ± 0.2 | 6.7 ± 1.7 | 6.7 ± 1.7 | 0.6 ± 0.0 | 0.8 ± 0.2 |
| OV-2 | 5.0 ± 0.0 | 5.0 ± 0.0 | 1.8 ± 0.7 | 0.2 ± 0.1 | >20.0 | >20.0 | ≥20.0 | ≥20.0 | >20.0 | >20.0 | >20.0 | >20.0 |
| OV-3 | 1.7 ± 0.4 | 1.7 ± 0.4 | 0.8 ± 0.2 | 0.8 ± 0.2 | 5.0 ± 0.0 | 5.0 ± 0.0 | 3.3 ± 0.8 | 4.2 ± 0.8 | 3.3 ± 0.8 | 3.3 ± 0.8 | 1.3 ± 0.0 | 2.5 ± 1.3 |
| OV-4 | 1.3 ± 0.0 | 1.3 ± 0.0 | 1.7 ± 0.4 | 1.3 ± 0.0 | 3.3 ± 0.8 | 2.5 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 1.0 ± 0.2 | 1.0 ± 0.2 |
| OV-5 | 0.8 ± 0.2 | 1.0 ± 0.2 | 8.3 ± 5.8 | 2.5 ± 0.0 | 2.5 ± 0.0 | 2.5 ± 0.0 | 11.7 ± 4.4 | 2.5 ± 0.0 | 2.1 ± 0.4 | 2.1 ± 0.4 | 1.3 ± 0.0 | 1.3 ± 0.0 |
| OV-6 | 1.3 ± 0.0 | 1.3 ± 0.0 | 5.0 ± 2.5 | 1.9 ± 0.6 | 2.5 ± 0.0 | 2.5 ± 0.0 | 2.1 ± 0.4 | 1.3 ± 0.0 | 5.0 ± 0.0 | 6.7 ± 1.7 | 2.1 ± 0.4 | 2.1 ± 0.4 |
| OV-7 | 1.5 ± 0.6 | 1.3 ± 0.0 | 3.3 ± 0.8 | 2.7 ± 1.3 | 3.3 ± 0.8 | 3.3 ± 0.8 | 7.5 ± 2.5 | 2.5 ± 0.0 | 5.0 ± 0.0 | 5.0 ± 0.0 | 1.3 ± 0.0 | 2.5 ± 1.3 |
| OV-8 | 5.0 ± 0.0 | >20.0 | 6.7 ± 1.7 | 5.0 ± 0.0 | 16.7 ± 3.3 | 16.7 ± 3.3 | ≥20.0 | >20.0 | >20.0 | ≥20.0 | 10.0 ± 5.0 | 15.0 ± 4.1 |
| OV-9 | 2.5 ± 0.0 | 5.0 ± 0.0 | 2.5 ± 0.0 | 4.2 ± 0.8 | 1.3 ± 0.0 | 1.3 ± 0.0 | 5.8 ± 2.2 | 5.8 ± 2.2 | >20.0 | ≥20.0 | 1.3 ± 0.0 | 1.7 ± 0.4 |
| OV-10 | 10.0 ± 0.0 | >20.0 | 4.2 ± 0.8 | 4.2 ± 0.8 | 6.7 ± 1.7 | 6.7 ± 1.7 | >20.0 | ≥20.0 | >20.0 | >20.0 | 15.0 ± 4.1 | 15.0 ± 4.1 |
| OV-11 | 3.3 ± 0.8 | 5.0 ± 0.0 | 1.7 ± 0.4 | 1.7 ± 0.4 | 4.2 ± 0.8 | 4.2 ± 0.8 | 5.0 ± 2.5 | 5.0 ± 2.5 | 20.0 ± 0.0 | 20.0 ± 0.0 | 5.0 ± 2.5 | 5.0 ± 2.5 |
| OV-12 | 8.3 ± 1.7 | >20.0 | 4.6 ± 2.7 | 4.6 ± 2.7 | 4.2 ± 0.8 | 4.2 ± 0.8 | 20.0 ± 0.0 | 20.0 ± 0.0 | >20.0 | >20.0 | ≥20.0 | ≥20.0 |
| OV-13 | >20.0 | >20.0 | 10.4 ± 5.4 | 7.1 ± 2.9 | 6.7 ± 1.7 | 6.7 ± 1.7 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 |
| OV-14 | 16.7 ± 3.3 | >20.0 | 8.3 ± 5.8 | 9.2 ± 5.5 | 8.3 ± 1.7 | 8.3 ± 1.7 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 |
| OV-15 | >20.0 | >20.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | ≥20.0 | >20.0 |
| OV-16 | ≥20.0 | >20.0 | 13.3 ± 3.3 | 13.3 ± 3.3 | 20.0 ± 0.0 | 20.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 |
|
Klebsiella pneumoniae
|
P. aeruginosa PAO1
|
Corynebacterium pseudotuberculosis ATCC 19410
|
Staphylococcus aureus
|
A. fumigatus
|
Hemolytic activity (%) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | |
| 1.3 ± 0.0 | 1.3 ± 0.0 | 4.2 ± 0.8 | 5.0 ± 2.5 | >20.0 | >20.0 | 5.0 ± 0.0 | 4.2 ± 0.8 | ND | ND | 100.0 ± 0.01 |
| 0.8 ± 0.2 | 0.8 ± 0.2 | 2.1 ± 0.4 | 2.5 ± 0.0 | >20.0 | >20.0 | 15.0 ± 5.0 | >20.0 | 10.0 ± 5.0 | 20.0 ± 0.0 | 8.1 ± 0.01 |
| >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | >20.0 | 6.7 ± 1.7 | 10.0 ± 0.0 | 6.2 ± 0.01 |
| 2.5 ± 0.0 | 2.5 ± 0.0 | 2.5 ± 0.0 | 5.0 ± 2.5 | >20.0 | >20.0 | 2.5 ± 0.0 | 2.5 ± 0.0 | 8.3 ± 1.7 | 16.7 ± 3.3 | 9.4 ± 0.01 |
| 10.0 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | >20.0 | >20.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 5.0 ± 0.0 | 10.0 ± 0.0 | ND |
| 1.7 ± 0.4 | 1.3 ± 0.0 | 2.1 ± 0.4 | 3.3 ± 0.8 | ≥20.0 | ≥20.0 | 4.2 ± 0.8 | 5.0 ± 0.0 | 6.7 ± 1.7 | 10.0 ± 0.0 | 8.0 ± 0.01 |
| 1.3 ± 0.0 | 1.3 ± 0.0 | 2.5 ± 0.0 | 2.5 ± 0.0 | ≥20.0 | >20.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 13.3 ± 3.3 | 16.7 ± 3.3 | ND |
| 1.7 ± 0.4 | 4.6 ± 2.7 | 4.2 ± 0.8 | 4.2 ± 0.8 | ≥20.0 | >20.0 | 10.0 ± 0.0 | 10.0 ± 0.0 | 8.3 ± 1.7 | 13.3 ± 3.3 | ND |
| ≥20.0 | ≥20.0 | 3.3 ± 0.8 | 5.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | 33.3 ± 6.7 | 40.0 ± 0.0 | 3.3 ± 0.01 |
| 10.0 ± 0.0 | 20.0 ± 0.0 | 2.5 ± 0.0 | 2.5 ± 0.0 | ≥20.0 | ≥20.0 | 10.0 ± 0.0 | ≥20.0 | 10.0 ± 0.0 | 23.3 ± 8.8 | ND |
| >20.0 | >20.0 | 5.0 ± 0.0 | 10.0 ± 0.0 | ≥20.0 | >20.0 | >20.0 | >20.0 | 40.0 ± 0.0 | >40.0 | 3.7 ± 0.01 |
| 6.7 ± 1.7 | 8.3 ± 1.7 | 1.7 ± 0.4 | 2.5 ± 0.0 | ≥20.0 | ≥20.0 | 10.0 ± 0.0 | ≥20.0 | 8.3 ± 1.7 | 8.3 ± 1.7 | 7.6 ± 0.01 |
| >20.0 | >20.0 | 0.6 ± 0.0 | 1.3 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | 5.8 ± 2.2 | 16.7 ± 3.3 | 2.4 ± 0.01 |
| ≥20.0 | ≥20.0 | 1.7 ± 0.4 | 2.5 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | 26.7 ± 6.7 | 26.7 ± 6.7 | 2.5 ± 0.01 |
| 15.0 ± 5.0 | 16.7 ± 3.3 | 20.0 ± 0.0 | 20.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | 20.0 ± 0.0 | 20.0 ± 0.0 | 2.5 ± 0.01 |
| 13.3 ± 3.3 | ≥20.0 | 5.0 ± 0.0 | 10.0 ± 0.0 | >20.0 | >20.0 | >20.0 | >20.0 | 40.0 ± 0.0 | >40.0 | 2.5 ± 0.01 |
| 11.7 ± 4.4 | 13.3 ± 3.3 | 10.0 ± 0.0 | 13.3 ± 3.3 | >20.0 | >20.0 | >20.0 | >20.0 | 40.0 ± 0.0 | ≥40.0 | 2.9 ± 0.01 |
a
Amino acid sequences are shown in Table 1. MICs and MBCs are shown in micrograms per milliliter as means ± standard errors of the means of three replications. OV, ovispirin; ND, not determined.