Fig. 4. CAO attenuated H2O2-induced cellular redox status imbalance. After pre-treatment with CAO (10 and 100 μM) for 24 h, HepG2 cells were then incubated with or without 300 μM H2O2 for another 4 h. (A) CAT activity in cells. (B) MDA level in cells. (C) SOD activity in cells. (D) GSH level in cells. (E) Images of ROS generation by 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) staining (scale bars: 50 μm). (F) Measurement of intracellular ROS level using flow cytometry. Data were expressed as mean ± SD, n = 6. One-way ANOVA was performed to test for statistical significance, p < 0.05.
