Fig. 6. Effect of CAO on the expression of P-p38 in HepG2 cells in response to H₂O₂. The cells were incubated with 100 μM CAO for 24 h with or without pretreatment with 10 μM SB203580 for 2 h before 300 μM H₂O₂ incubation for 4 h. (A) Effects of p38 inhibitor SB203580 on the cell viability. (B) Measurement of protein phosphorylation by western blot analysis. (C) Quantitative analysis was performed by measuring intensity relative to the untreated control. Each value represents the mean ± SD for three independent experiments. One-way ANOVA was performed to test for statistical significance, p < 0.05.