Methods for the refined purification of tea saponins.
| Refined method | Precipitating agent | Purity (%) | References |
|---|---|---|---|
| Two-phase system extraction | 20% (NH4)2SO4 + 23% n-propyl alcohol (w/w) | 78.12 | 63 |
| Reversed-phase column chromatography | Methanol | 95 | 64 |
| Ethanol | 99.12 | 55 | |
| Macroporous resin | AB-8 | >80–96.13% | 39 and 65–67 |
| XR910X | 94.26 | 68 | |
| D101 | 60.14 | 69 | |
| Molecularly imprinted polymers | Acrylamide–β-cyclodextrin | 89 | 70 |
| Flocculation + ultrafiltration | Chitosan | 85 | 36 |
| Ultrafiltration | 91 | 71 | |
| Precipitation | Acetone | 72.06 to >93 | 50, 72 and 73 |
| Cholesterol | >98.5 | 74 | |
| Ether | 97.9 | 75 | |
| Recrystallization | Ethanol–hydrochloric acid | 81.8 | 76 |