Fig. 1.

a RNA-seq and immunofluorescence. RNA-seq indicated the presence of various immune cells in the human cornea; notably CD8+ T cells, Th17 cells, dendritic cells, and macrophages (n = 3 individual corneas). b Besides PTPRC (CD45), macrophage-specific marker genes, such as ITGAM (CD11b), CD14, and CD74, can be detected by RNA-seq. c Immunofluorescence confirms the presence of M1 and M2 macrophages, with M1 macrophages appearing to be more abundant than M2 macrophages (representative images from 3 individual human corneas). RNA-seq, RNA-sequencing; M1, pro-inflammatory macrophages; M2, regulatory macrophages.