TABLE 1.

Summary data (OD values) on inhibition of S. epidermidis biofilm formation^a

No. of triplicate evaluation	Mean OD570 value (mean % inhibition) for various treatment regimens
	A	B	C	D (reference)
Run 1	0.179 (65.4)	0.000 (99.9)	0.062 (88.0)	0.517
Run 2	0.383 (35.3)	0.001 (99.9)	0.329 (44.4)
Run 3	0.469 (46.8)	0.001 (99.9)	0.225 (74.5)
Total mean (SEM) % inhibition	49.2 (8.8)b	99.9 (0.04)b	69.1 (12.9)

^aAn OD₅₇₀ ≥0.1 represents biofilm production. S. epidermidis suspensions that gave a final inoculum size that ranged between 5 × 10⁵ and 1 × 10⁶ CFU/ml were introduced to one of the four treatment regimens and were incubated with shaking (150 rpm) for 24 h at 37°C. Control wells containing CAMHB only, but no bacteria, were also included. The average ODs derived from these provided the zero values, which enabled circulation of the relative OD₅₇₀ values. 

^bThe statistical significance of the relative percentage of biofilm inhibition was determined, and the difference between treatments A and B was significant (P = 0.022). However, only treatment B prevented biofilm formation. Comparisons of the means among the groups were done by one-way analysis of variance by Bonferroni multiple separation tests. Statistical analyses were performed with Stata software (version 7; Stata Corp., College Station, Tex.).