TABLE 4.
Effect of salicylate in combination with one-half the MBEC of vancomycin on S. epidermidis biofilms
| No. of duplicate evaluation | Inoculum size (reference biofilm cell eradication)a | No. of viable cells recovered (no. of CFU/bead) for treatment regimen:
|
|||
|---|---|---|---|---|---|
| A | B | C | D (reference biofilm growth inhibition) | ||
| Run 1 | 9.5 × 105 | 1.0 × 106 (−, 0)b | 1.3 × 101 (−, 99.9) | 1.0 × 106 (++, 0) | 9.0 × 106 (++++) |
| Run 2 | 5.0 × 105 | 9.0 × 105 (−, 0) | 8.3 × 101 (−, 99.98) | 2.0 × 106 (++, 0) | 1.1 × 107 (++++) |
| Run 3 | 1.5 × 106 | 1.3 × 106 (−, 13.33) | 9.5 × 102 (−, 99.94) | 6.5 × 106 (++, 0) | (++++) |
| Mean no. of log10 CFU/bead | 5.99 | 6.04 | 2.54 | 6.51 | 7.15 |
| Mean % eradication of biofilm cellsc | <10 | >99.9 | 0 | NAd | |
| Mean % inhibition of biofilm growthe | 92 | NA | 85 | ||
For each evaluation, a representative inoculum size was determined as described in the text. This value served as a reference. S. epidermidis biofilms growing on beads (5 × 105 to 1.5 × 106CFU/bead) were subjected to three independent test evaluations.
Information in parentheses represents the growth turbidity (++++ or ++, turbid [visible growth in tube]; (−) no visible growth), percent eradication.
The statistical significance of the percent reduction of the inoculum in size medium (CAMHB) only was determined, and the percent reduction from treatment B was significantly greater than that from treatment A or C (P = 0.000). Comparison of the means among the groups was tested by one-way analysis of variance by Bonferroni multiple separation tests. Statistical analyses were performed with Stata software (version 7; Stata. Corp.).
NA, not applicable (therefore, no further assessment was done).
Relative to reference treatment D.