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. 2022 Apr 25;3:861598. doi: 10.3389/fpain.2022.861598

Figure 5.

Figure 5

Injection of CGRP into the MN induced plantar tactile hypersensitivity in the contralateral hind paw. Plantar tactile sensitivity was assessed with injection of PBS or CGRP (1 μg/200 nl) into the right MN of C57BL/6J mice via cannulas. Data are from three independent experiments. (A) The individual thresholds of left hind paws for all mice (left) (PBS: n = 17; CGRP: n = 26), female mice (middle) (PBS: n = 8; CGRP: n = 14), and male mice (right) (PBS: n = 9; CGRP: n = 12). (B) Comparison of changes in withdrawal thresholds of left hind paws between CGRP-treated female and CGRP-treated male mice. The change in thresholds was measured by subtracting respective baseline from CGRP treatment measurements. (C) The individual thresholds of right hind paws for all mice (left) (PBS: n = 17; CGRP: n = 26), female mice (middle) (PBS: n = 8; CGRP: n = 14), and male mice (right) (PBS: n = 9; CGRP: n = 12). (D) Comparison of changes in withdrawal thresholds of right hind paws between CGRP-treated female and CGRP-treated male mice. The mean ± SEM 50% thresholds are presented. Statistics are described in Supplementary Table 1. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.