Figure 6.

Injection of CGRP into the MN induced nociceptive squinting behavior. (A) Mean pixel area over 5-min testing period for all mice without treatment (as baseline), with injection of PBS (left) or CGRP (middle; 1 μg/200 nl) into the right MN of C57BL/6J mice via cannulas. Right panel is the mean pixel area over 5-min testing period for individual mice (PBS: n = 30; CGRP: n = 27). Data from A separated as female (B) and male (C). Data are from two independent experiments and one crossover treatment experiment. (B) Mean pixel area over 5-min testing period for female mice (left and middle) and mean pixel area over 5-min testing period for individual female mice (right; PBS: n = 17; CGRP: n = 14). (C) Mean pixel area over 5-min testing period for male mice (left and middle) and mean pixel area over 5-min testing period for individual male mice (right; PBS: n = 13; CGRP: n = 13). Data are the mean ± SEM. Statistics are described in Supplementary Table 1. *p ≤ 0.05, **p ≤ 0.01.