FIG. 2.

Genetic structure of the three open reading frames (ORFs) from the sequenced fragments of E. faecium UW786 and UW1965. Putative amino acid sequence of ORF1 to -3 is shown in accordance with results from databank screens (100% identical to parts of GenBank accession number AF299292). Restriction endonuclease sites for DdeI (CTNAG) are shown. Small black arrows indicate the binding sites for the two internal primers, aadE-1 and aphA-2. The start codon of ORF2 (sat4) overlaps with the stop codon of ORF1 (aadE). The complete nucleotide composition and putative protein sequences are given in GenBank under accession number AF330699.