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. Author manuscript; available in PMC: 2022 May 9.
Published in final edited form as: Cell Host Microbe. 2022 Jan 19;30(2):260–272.e5. doi: 10.1016/j.chom.2021.12.008

Figure 4: Pre-exposure of the gut microbiota to ciprofloxacin in vivo results in differential invasion of S. Typhimurium in vitro.

Figure 4:

A) Experimental setup for in vitro challenge with S. Typhimurium. SICs passaged in BHI from pre-, peak, residual, and post-treatment humanized mouse fecal inocula were revived after freezing and passaged twice in BHI. SICs were mixed with S. Typhimurium and S. Typhimurium levels were quantified after 48 h of growth.

B) SICs derived from mice treated with ciprofloxacin are more susceptible to S. Typhimurium. Colonies of S. Typhimurium SL1344 after 48 h of growth with SICs diluted 1:104 and grown aerobically on LB+streptomycin.

C) Single-cell quantification of mCherry-tagged S. Typhimurium 14028s after co-culture with SICs derived from pre- and residual-treatment mice fecal inocula. p-value is from a Student’s two-sided t-test; n=3.