Precision and accuracy data of back calculated concentrations of calibration standard samples of DNP in rat plasma (n = 6)^a.

Component	Linear equation and coefficient of regression	Nominal concentration (ng mL−1)	Measured concentration (Mean ± SD, ng mL−1)	Precision (% CV)	Accuracy (% bias)
Plasma	y = 0.000384x − 0.0088, r2 = 0.9999	50	51.87 ± 3.21	6.19	3.74
		100	95.30 ± 4.57	4.80	−4.70
		300	304.26 ± 16.99	5.58	1.42
		1000	999.58 ± 2.96	0.30	−0.04
		2000	1983.08 ± 12.60	0.64	−0.85
		3500	3464.12 ± 5.27	0.15	−1.03
		5000	5042.00 ± 8.58	0.17	0.84
Brain	y = 0.0003x − 0.0058, r2 = 0.9998	50	55.26 ± 1.15	2.08	10.52
		300	289.54 ± 15.48	5.35	−3.49
		1000	939.40 ± 87.62	9.33	−6.06
		2000	1950.68 ± 13.60	0.70	−2.47
		3500	3335.07 ± 30.75	9.18	−4.71
		5000	4872.22 ± 19.75	0.41	−2.56
Heart	y = 0.0003x − 0.0075, r2 = 0.9991	50	56.24 ± 6.54	11.63	12.48
		300	314.36 ± 1.10	0.35	4.79
		1000	1011.78 ± 18.88	1.87	1.18
		2000	2147.01 ± 51.93	2.42	7.35
		3500	3547.65 ± 4.37	0.12	1.36
		5000	5262.01 ± 43.58	0.83	5.24
Lungs	y = 0.0003x − 0.0099, r2 = 0.9999	50	56.78 ± 4.23	7.45	13.56
		300	316.62 ± 28.83	9.11	5.54
		1000	1041.39 ± 93.03	8.93	4.14
		2000	2168.05 ± 106.81	4.93	8.40
		3500	3749.94 ± 206.25	5.50	7.14
		5000	5372.00 ± 291.26	5.42	7.44
Spleen	y = 0.0003x + 0.0089, r2 = 0.9996	50	55.31 ± 3.32	6.00	10.62
		300	289.15 ± 8.66	2.99	−3.62
		1000	1049.53 ± 80.29	7.65	4.95
		2000	2148.97 ± 146.01	6.79	7.44
		3500	3600.13 ± 269.69	7.49	2.86
		5000	5247.77 ± 32.88	0.63	4.96
Kidney	y = 0.0003x − 0.0008, r2 = 0.9995	50	46.42 ± 4.12	8.88	−7.16
		300	309.21 ± 3.51	1.14	3.07
		1000	1050.37 ± 85.23	8.11	5.04
		2000	2243.01 ± 223.27	9.95	12.15
		3500	3804.79 ± 163.19	4.29	8.71
		5000	5355.65 ± 51.11	0.95	7.11
Liver	y = 0.0003x − 0.0126, r2 = 0.9999	50	52.94 ± 1.68	3.17	5.88
		300	281.24 ± 25.88	9.20	−6.25
		1000	935.92 ± 29.60	3.16	−6.41
		2000	1880.22 ± 135.81	7.22	−5.99
		3500	3240.65 ± 18.66	0.58	−7.41
		5000	4703.54 ± 5.48	0.12	−5.93

^aSD, standard deviation; CV, coefficient of variation.