Table 1.
The quality of data output.
| Sample | Raw reads | Clean reads | Raw bases (G) | Clean bases (G) | Error (%) | Q20 (%) | Q30 (%) | GC (%) |
|---|---|---|---|---|---|---|---|---|
| AR1 | 22995633 | 22373634 | 5.75 | 5.59 | 0.06 | 91.96 | 85.92 | 50.48 |
| AR2 | 28353082 | 27932670 | 7.09 | 6.98 | 0.04 | 94.19 | 89.22 | 50.56 |
| AR3 | 21275181 | 21220183 | 5.32 | 5.31 | 0.06 | 92.59 | 86.52 | 50.05 |
| ER1 | 23576166 | 23185535 | 5.89 | 5.8 | 0.04 | 94.19 | 89.28 | 49.38 |
| ER2 | 18195848 | 18136753 | 4.55 | 4.53 | 0.04 | 96.15 | 92.00 | 48.24 |
| ER3 | 21778235 | 21267590 | 5.44 | 5.32 | 0.06 | 91.91 | 85.81 | 49.13 |
Raw reads: the original sequence data was counted, four behaviors per unit, statistics of the number of sequencing sequences of each file.
Clean reads: the calculation method was the same as raw reads, but the statistical files were filtered sequencing data.
Raw bases (G): the number of original sequence data was multiplied by the length of the sequencing sequence, unit: G (data).
Clean bases (G): the number of filtered sequences multiplied by the length of the sequence, unit: G (data).
Error (%): base error rate. Q20, Q30 (%): the percentage of bases with Phred values > 20 and 30 to the total bases.
GC (%): the percentage of the total number of bases G and C to the total number of bases.