TABLE 1.
| Strain | Location of mutation for indicated gene
|
|||
|---|---|---|---|---|
| GrlA | GrlB | GyrA | GyrB | |
| Third-step mutant strains | ||||
| 3(756)6-1 | 116 (Ala→Glu) | —c | 84 (Ser→Leu) | — |
| 3(756)6-2 | — | — | 84 (Ser→Leu) | — |
| 3(756)6-3 | 157 (Pro→Leu) | — | — | — |
| 3(756)10-1 | — | — | 84 (Ser→Leu) | — |
| 3(756)10-2 | — | — | 84 (Ser→Leu) | — |
| 3(756)10-3 | — | — | 84 (Ser→Leu) | — |
| Fourth-step mutant strains | ||||
| 4(756)6-1 | — | — | 84 (Ser→Leu) | — |
| 4(756)6-2 | 80 (Ser→Phe) | — | 84 (Ser→Leu) | — |
| 4(756)10-1 | — | — | 84 (Ser→Leu) | — |
| 4(756)10-3 | 80 (Ser→Phe) | — | 84 (Ser→Leu) | — |
| Fifth-step mutant strains | ||||
| 5(756)6-1 | — | 472 (Glu→Lys)d | 84 (Ser→Leu) | — |
| 5(756)6-2 | — | 472 (Glu→Lys) | 84 (Ser→Leu) | — |
| 5(756)10-1 | — | — | 84 (Ser→Leu) | — |
| 5(756)10-3 | — | — | 84 (Ser→Leu) | — |
a
First- and second-step BMS-284756-selected mutants had no changes in the QRDRs of the four genes sequenced.
b
Four ciprofloxacin-selected mutants, 1C6(1)-1, 2C6(1)-1, 3C6(1)-1, and 4C6(1)-1, were sequenced. They all had only one QRDR mutation, grlA (A116E).
c
The designation — indicates no change.
d
Mutations not previously reported.