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Fig. 2. Difference electron density of 1 in the galectin-1 binding site. Difference electron density calculated from refinement with the ligand (stick representation) omitted from the model (|Fo| − |Fc| αcalc; grey mesh, contoured at 3σ) and the protein represented by a grey solvent-accessible surface. The narrow pocket accommodating one of the thiophene rings is indicated with a yellow arrow.