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. 2022 Apr 25;10:843297. doi: 10.3389/fcell.2022.843297

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Copyright © 2022 Tang, Liu, Zhu, Chen, Sun, Zhang, Zhao, Wu, Fei, Ye and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of ferroptosis-associated gene markers. (A): validation of gene marker mRNA levels in the hippocampus for ferroptosis by qRT-PCR. (B,C): Validation of GPX4 and SLC7A11 protein expression level in the hippocampus by western blot. n = 3–6 rats/group, *p < 0.05 vs sham group. The student’s t test was used for comparisons.