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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Nat Genet. 2021 Aug 12;53(9):1360–1372. doi: 10.1038/s41588-021-00906-y

Fig. 5 ∣. Expression of aldosterone synthase (CYP11B2) and 11β-hydroxylase (CYP11B1) in GNA11/Q and CTNNB1 double-mutant APAs.

Fig. 5 ∣

a, Quantitative PCR analysis of CYP11B1 and CYP11B2 mRNA expression demonstrated that double-mutant APAs have a lower CYP11B1/CYP11B2 mRNA expression ratio compared to CTNNB1 single-mutant APAs or APAs wild type for CTNNB1 and GNA11/Q (CTNNB1-negative APA) (P = 0.00004 by one-way Kruskal–Wallis test, χ2(2) = 20.23; P values from Dunn’s multiple comparisons test are as indicated). Results are expressed as fold change from CTNNB1 wild-type APAs (CTNNB1-negative APA). Error bars presents mean ± s.e.m. The exact sample number (n), as indicated below the x axis, represents biologically independent samples. Squares, males; circles, females; red symbols, double mutants; blue symbols, KCNJ5 mutants; black symbols, KCNJ5 wild type. b, Immunohistochemistry of CYP11B2 and CYP11B1 in the UK/Irish cohort using the primary antibody anti-CYP11B2 and anti-CYP11B1 no. MABS502, clone 80-7. The histotype of high CYP11B2 protein expression and low CYP11B1 expression was apparent, correlating with the low CYP11B1/CYP11B2 mRNA expression seen in a. Scale bars, 2.5 mm.