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. 2022 May 5;221(6):e202105112. doi: 10.1083/jcb.202105112

Figure 8.

Figure 8.

The Salmonella effector protein SopF blocks LAP and non-canonical autophagy. (a) Confocal images of GFP-hLC3A expressing RAW264.7 cells transfected with mCherry-SopF or empty vector and stimulated with OPZ for 25 min. Asterisks denote phagosomes and arrows mark GFP-LC3A positive phagosomes and dashed line marks outline of the cell. Scale bar, 5 μm. (b) Quantification of the percentage of phagocytosing mCherry-SopF or empty vector expressing cells that contain GFP-hLC3A positive phagosomes following OPZ stimulation. Data represent the mean ± SEM from three independent experiments. ***, P < 0.0003, unpaired t test. (c) Representative confocal DIC images of NBT/formazan deposits in phagosomes from empty vector and mCherry-SopF expressing RAW264.7 cells. Scale bar, 5 μm. (d) WT MCF10A cells expressing mCherry-SopF or empty vector were treated with SaliP (2.5 μM) or monensin (100 μM) for 1 h. Following fractionation, input, membrane, and cytosol fractions were probed for ATP6V1A, ATP6V0d1, and mCherry by Western blotting. Data representative of two repeats. Source data are available for this figure: SourceData F8.