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Time-lapse spinning-disk confocal microscopy showing phagocytosis of OPZ in RAW264.7 cells expressing GFP-hLC3A and RFP-Rab7. Sequential recruitment of RFP-Rab7 and hGFP-LC3A to phagosomes is observed. Image stacks acquired every 30 s. Movie plays 4 frames/s; time, min:s. Related to Fig. 1 a.
