FIG. 4.

(A) ELISA of TEM-1 β-lactamase binding to immobilized Biotin-MiniBLIP peptide. A total of 10 μg of Biotin-MiniBLIP per ml was immobilized on NeutrAvidin-coated microtiter wells. After extensive washing to remove unbound protein, TEM-1 β-lactamase and MBP were tested for their levels of binding to the immobilized peptide. Bound TEM-1 β-lactamase was assayed with a polyclonal sera directed toward the TEM-1 β-lactamase. Bound MBP was assayed with a polyclonal sera directed toward MBP. (B) As a control, the sera with anti-β-lactamase and anti-MBP antibodies were tested for their reactivities against the indicated proteins. A total of 10 μg of each protein per ml was immobilized in microtiter wells and was then probed with polyclonal sera directed toward either the TEM-1 β-lactamase (B, left panel) or MBP (B, right panel). All datum points are the averages for two samples. An asterisk indicates that no detectible ELISA signal was observed for the samples.